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Bioengineering
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酵素の固定化と安定化のための疎水性の塩で修飾ナフィオン
JoVE Journal
Bioengineering
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JoVE Journal
Bioengineering
Hydrophobic Salt-modified Nafion for Enzyme Immobilization and Stabilization
Please note that all translations are automatically generated.
Click here for the English version.
酵素の固定化と安定化のための疎水性の塩で修飾ナフィオン
DOI:
10.3791/3949-v
•
11:16 min
•
July 11, 2012
•
Shannon Meredith
,
Shuai Xu
,
Matthew T. Meredith
,
Shelley D. Minteer
1
Departments of Chemistry and Materials Science and Engineering
,
University of Utah
Chapters
00:05
Title
01:47
Modification of Nafion with Quaternary Ammonium Salts
04:55
Immobilization of Enzymes into TBAB-modified Nafion for Activity Assays
05:56
Assay of Immobilized NAD-dependent Dehydrogenase
07:05
Assay of Immobilized PQQ-dependent Dehydrogenases
08:15
Assay of Immobilized Glucose Oxidase
09:11
Results: Activity of Enzymes Immobilized on Various Modified Nafion Films
11:00
Conclusion
Summary
Automatic Translation
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Automatic Translation
この資料では、疎水性修飾ナフィオン酵素固定化膜方法と膜内のタンパク質および/または酵素を固定化とその特異的活性をテストするを合成するための手順を説明します。
Tags
Hydrophobic Salt-modified Nafion
Enzyme Immobilization
Enzyme Stabilization
Biocatalysis
Biosensors
Biofuel Cells
Polymer Matrix
Polyamines
Polyalcohols
Micelles
Minteer Group
Nafion
Alkyl Ammonium Salts
Tetrabutylammonium Bromide (TBAB)
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