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酵素の固定化と安定化のための疎水性の塩で修飾ナフィオン
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Hydrophobic Salt-modified Nafion for Enzyme Immobilization and Stabilization
DOI:

11:16 min

July 11, 2012

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Chapters

  • 00:05Title
  • 01:47Modification of Nafion with Quaternary Ammonium Salts
  • 04:55Immobilization of Enzymes into TBAB-modified Nafion for Activity Assays
  • 05:56Assay of Immobilized NAD-dependent Dehydrogenase
  • 07:05Assay of Immobilized PQQ-dependent Dehydrogenases
  • 08:15Assay of Immobilized Glucose Oxidase
  • 09:11Results: Activity of Enzymes Immobilized on Various Modified Nafion Films
  • 11:00Conclusion

Summary

Automatic Translation

この資料では、疎水性修飾ナフィオン酵素固定化膜方法と膜内のタンパク質および/または酵素を固定化とその特異的活性をテストするを合成するための手順を説明します。

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