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一个系统<em>体外</em培养的胚胎胰腺

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Biology

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JoVE Journal Biology

A System for ex vivo Culturing of Embryonic Pancreas

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一个系统<em>体外</em培养的胚胎胰腺

DOI:

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10:51 min

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August 27, 2012

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Kristin M. Petzold, Francesca M. Spagnoli

¹Molecular and Cellular Basis of Embryonic Development,Max-Delbrück-Center for Molecular Medicine

Chapters

00:05Title
01:36Dissection of Dorsal Pancreatic Bud from E11.5-E12.5 Mouse Embryos
03:53Plating and Culture of Pancreatic Explants
05:01Whole-mount Immunofluorescence Staining Protocol for Pancreatic Explants
07:14Live-cell Imaging Protocol of Pancreatic Explants
07:55Results: Representative Images of Cultured Pancreatic Explant Sections
10:28Conclusion

Summary

Automatic Translation

在这里，我们描述了一个小鼠胚胎胰腺分离，培养和操纵方法。这是一个极好的<em>体外</em系统为研究胰腺发育的各个方面，包括形态，分化和生长。胰芽外植体可以培养数天，并用于在一个范围内的不同的应用程序，包括整体安装免疫荧光和实时成像。

Tags

Ex Vivo Culturing Embryonic Pancreas Pancreatic Organogenesis Early Pancreas Formation Diabetes Pancreatic Cancer Cell-replacement Therapies Embryogenesis Dorsal And Ventral Foregut Endoderm Epithelial Buds Proliferation Branching Differentiation Epithelial Remodeling Cell Positioning Branching Morphogenesis

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