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建立真核微生物的富集培养从化学分层南极湖和固碳潜力评估
JoVE Journal
Biology
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JoVE Journal Biology
Establishment of Microbial Eukaryotic Enrichment Cultures from a Chemically Stratified Antarctic Lake and Assessment of Carbon Fixation Potential
DOI:

14:38 min

April 20, 2012

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Chapters

  • 00:05Title
  • 02:03Sample Acquisition
  • 04:24Development of Enrichment Cultures
  • 06:42Cell Lysate Extraction from Filtered Enrichments
  • 08:44RubisCO Carboxylase Activity Filter Assay
  • 11:17Results: Analysis of Novel Protists from Lake Bonney, Antarctica
  • 13:55Conclusion

Summary

Automatic Translation

微生物的真核生物都在永久性冰雪覆盖的南极湖泊源的光合衍生的碳和顶级掠食性物种。本报告介绍了富集培养的方法分离代谢多功能微生物真核生物,从南极湖,湖波尼,使用核酮糖1,5 - 二磷酸羧化酶加氧酶(Rubisco活化酶)活性放射性同位素检测和评估无机碳固定潜力。

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