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使用机器人在油脂中间相高通量膜蛋白的结晶
JoVE Journal
Biology
Author Produced
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JoVE Journal
Biology
Use of a Robot for High-throughput Crystallization of Membrane Proteins in Lipidic Mesophases
Please note that all translations are automatically generated.
Click here for the English version.
使用机器人在油脂中间相高通量膜蛋白的结晶
DOI:
10.3791/4000-v
•
20:21 min
•
September 01, 2012
•
Dianfan Li
,
Coilín Boland
,
Kilian Walsh
,
Martin Caffrey
1
Membrane Structural and Functional Biology Group, Schools of Medicine and Biochemistry & Immunology
,
Trinity College Dublin
Chapters
00:00
Title
01:35
Crystallisation Plate Preparation
03:54
Robot I:
In Meso
Robot
08:48
Priming the Syringe
09:27
Running the Robot
11:14
Sealing the Plate
13:13
Robot 2: Mosquito LCP
18:00
Robot 3: Gryphon LCP
19:55
Concluding Remarks
Summary
Automatic Translation
English (Original)
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Automatic Translation
本文中描述了一种机器人的方法,在使用大分子X-射线晶体结构的测定中使用的脂质中间相为高通量膜蛋白结晶。 3个机器人能处理粘性和粘蛋白载货中间相积分的方法进行了介绍。
Tags
Robot
High-throughput Crystallization
Membrane Proteins
Lipidic Mesophases
Macromolecular X-ray Crystallography
MX
Diffraction-quality Crystals
In Meso Method
Lipidic Cubic Phase (LCP) Method
Bicontinuous Mesophase
Toothpaste-like Consistency
Dispensing
Reproducible Manner
Small Volumes
Crystallization Wells
Manual Approach
Specialty Applications
Crystal Optimization
Derivatization
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