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准备从大鼠脾细胞T细胞生长因子
JoVE Journal
Biology
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JoVE Journal
Biology
Preparing T Cell Growth Factor from Rat Splenocytes
Please note that all translations are automatically generated.
Click here for the English version.
准备从大鼠脾细胞T细胞生长因子
DOI:
10.3791/402-v
•
18:27 min
•
October 31, 2007
•
Christine Beeton
,
K. George Chandy
1
Department of Physiology and Biophysics
,
University of California, Irvine (UCI)
Chapters
00:00
Title
00:06
Introduction
01:13
TCGF Secretion
03:22
Harvesting Spleens from Lewis Rats
05:15
Preparing Single Cell Suspensions from Rat Spleens
10:23
Lysis of Red Blood Cells
12:40
Preparing Splenocyte Culture
13:34
Elimination of the Conconavalin A
17:53
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们描述的T细胞生长因子在体外扩增抗原特异性大鼠T淋巴细胞线的准备。
Tags
T Cell Growth Factor
Rat Splenocytes
Antigen-specific T Cell Lines
Interleukin 2
Th1 Polarization
TCGF Preparation
Cytokines
Large-scale Production
Rat Splenocyte Culture Supernatants
Concanavalin A
Mitogen
Cytokine Production
Sterile Filtration
Storage At -20°C
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