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Journal
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Biology
/
补肾胶囊对糖尿病小鼠的胰岛移植
JoVE Journal
Biology
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JoVE Journal
Biology
Transplantation of Pancreatic Islets Into the Kidney Capsule of Diabetic Mice
Please note that all translations are automatically generated.
Click here for the English version.
补肾胶囊对糖尿病小鼠的胰岛移植
DOI:
10.3791/404-v
•
10:43 min
•
October 31, 2007
•
Gregory L. Szot
,
Pavel Koudria
,
Jeffrey A. Bluestone
1
Diabetes Center
,
University of California, San Francisco – UCSF
Chapters
00:01
Title
00:17
Introduction
01:07
Hand-Picking the Islets
03:18
Transferring Islets into P150 Tubing
05:17
Preparing the Mouse for Islet Transplant
05:59
Exposing the Kidney
07:16
Transplantation of Islets
10:01
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们的协议是干净,便捷地提供胰岛细胞或细胞小鼠肾囊下。集中使用,用于移植的肾囊下细胞最终油管颗粒细胞。这项技术的易用性,减少压力的细胞和鼠标。
Tags
Transplantation
Pancreatic Islets
Kidney Capsule
Diabetic Mice
Protocol
Cells
Delivery Tubing
PE50
Concentration
Pellets
Speed
Ease
Stress Reduction
Loading
Microcentrifuge Tube
Pipetteman
Pipette Tip
PE50 Tubing Attachment
Silicone Adapter Tubing
Settling
Dialing
Kink
15 ML Conical
Centrifuging
Anesthesia
Shaving
Cleaning
Incision
Kidney Exposure
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