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复制和β细胞功能评估Adenovirally转分离的啮齿动物胰岛
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Medicine
Assessing Replication and Beta Cell Function in Adenovirally-transduced Isolated Rodent Islets
Please note that all translations are automatically generated.
Click here for the English version.
复制和β细胞功能评估Adenovirally转分离的啮齿动物胰岛
DOI:
10.3791/4080-v
•
09:31 min
•
June 25, 2012
•
Patrick T. Fueger
2
,
Angelina M. Hernandez
,
Yi-Chun Chen
,
E. Scott Colvin
2
1
Department of Pediatrics
,
Indiana University School of Medicine
,
2
Department of Cellular & Integrative Physiology
,
Indiana University School of Medicine
Chapters
00:05
Title
00:58
Adenoviral Transduction and Culturing of Rat Islets
03:26
Insulin Secretion Assay
06:13
Thymidine Incorporation Assay
07:49
Beta Cells Overexpressing “Gene #6”
09:09
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议允许一个确定调节功能的β细胞质量的因素,找到治疗糖尿病的潜在的治疗靶点。该协议包括一个精简的方法来评估胰岛细胞复制和β细胞功能,在离体大鼠胰岛细胞与腺病毒基因表达操纵。
Tags
Replication
Beta Cell Function
Adenovirally-transduced Isolated Rodent Islets
Glucose Homeostasis
Insulin
Glucagon
Pancreatic Beta Cells
Alpha Cells
Functional Beta Cell Mass
Nutrient Load
Diabetes
Autoimmune Attack
Type 1 Diabetes
Type 2 Diabetes
Insulin Secretion
Molecular Pathways
Therapeutic Targets
Beta Cell Growth
Impairing Beta Cell Function
Oncogenes
Target Genes
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