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Chapters
- 00:05Title
- 02:03Haploid Cell Growth
- 03:08Conducting a Cross
- 04:43Harvesting Preparations
- 05:57Flow Cytometry
- 09:01Preparation for DeltaVision Microscopy
- 11:05Representative Analysis of Zygote Purification
- 14:27Conclusion
Per purificare zigoti di<em> S. cerevisiae</em>, Cellule aploidi di tipo opposto accoppiamento sono stati progettati per esprimere proteine fluorescenti rossi o verdi, co-incubate per permettere la formazione dello zigote, e frazionati con un protocollo basato citometria a flusso. Il altamente arricchito frazione consente la successiva "-omiche" studi, il recupero della progenie iniziale, e la ricerca sistematica della morfogenesi zigote.
Tags
Flow Cytometry-based PurificationS. Cerevisiae ZygotesHaploid CellsDiploid StatesMating TypeStable DiploidsMitotic DivisionsZygote FormationSoluble Mating FactorsReceptor-mediated Signaling CascadesCell-cell FusionModel For Progenitor Or Stem Cell FunctionBiochemistry Of Zygote FormationPurification Of Yeast ZygotesSedimentation PropertiesHypertonic Sorbitol
