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异型三维<em在体外</em>卵巢癌的发生和发展过程中的间质 - 上皮细胞相互作用模型
JoVE Journal
Medicine
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JoVE Journal Medicine
Heterotypic Three-dimensional In Vitro Modeling of Stromal-Epithelial Interactions During Ovarian Cancer Initiation and Progression
DOI:

12:42 min

August 28, 2012

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Chapters

  • 00:05Title
  • 01:54Primary NOF Cell Line: Overexpressing hTERT
  • 04:15PolyHEMA Coating of Cell Culture Dishes
  • 05:07Generating 3D Heterotypic Spheroids
  • 06:43Re-feeding of 3D Cultures
  • 07:58Processing for Imaging and Molecular Analyses
  • 11:36Conclusion

Summary

Automatic Translation

我们描述的方法建立<em在体外</em>异型三维模型,包括卵巢成纤维细胞和正常卵巢表面或卵巢癌上皮细胞。我们讨论了使用这些模型来研究间质 - 上皮细胞相互作用过程中发生卵巢癌的发展。

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