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异型三维<em在体外</em>卵巢癌的发生和发展过程中的间质 - 上皮细胞相互作用模型
JoVE Journal
Medicine
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JoVE Journal
Medicine
Heterotypic Three-dimensional
In Vitro
Modeling of Stromal-Epithelial Interactions During Ovarian Cancer Initiation and Progression
Please note that all translations are automatically generated.
Click here for the English version.
异型三维<em在体外</em>卵巢癌的发生和发展过程中的间质 - 上皮细胞相互作用模型
DOI:
10.3791/4206-v
•
12:42 min
•
August 28, 2012
•
Kate Lawrenson
,
Barbara Grun
,
Simon A. Gayther
1
Department of Preventive Medicine
,
University of Southern California
,
2
Institute for Women’s Health
,
University College London
Chapters
00:05
Title
01:54
Primary NOF Cell Line: Overexpressing hTERT
04:15
PolyHEMA Coating of Cell Culture Dishes
05:07
Generating 3D Heterotypic Spheroids
06:43
Re-feeding of 3D Cultures
07:58
Processing for Imaging and Molecular Analyses
11:36
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们描述的方法建立<em在体外</em>异型三维模型,包括卵巢成纤维细胞和正常卵巢表面或卵巢癌上皮细胞。我们讨论了使用这些模型来研究间质 - 上皮细胞相互作用过程中发生卵巢癌的发展。
Tags
Heterotypic Three-dimensional In Vitro Modeling
Stromal-epithelial Interactions
Ovarian Cancer Initiation
Ovarian Cancer Progression
Epithelial Ovarian Cancers
Gynecological Malignancy
Surgical Treatments
Platinum-based Chemotherapies
EOC Survival Rates
Stage I Tumors
Stage III/IV Disease
Mortality Rates
Molecular Genetic Basis
Biological Basis
Early Stage Disease Development
Microenvironment Role
Risk Factors For EOCs
Normal Ovary Co-culture
Ovarian Surface Epithelial Cells
Stromal Fibroblast Cells
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