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Chapters
- 00:05Title
- 01:54Primary NOF Cell Line: Overexpressing hTERT
- 04:15PolyHEMA Coating of Cell Culture Dishes
- 05:07Generating 3D Heterotypic Spheroids
- 06:43Re-feeding of 3D Cultures
- 07:58Processing for Imaging and Molecular Analyses
- 11:36Conclusion
Descriviamo metodologie per la creazione di<em> In vitro</em> Eterotipiche modelli tridimensionali comprendenti fibroblasti ovariche e di superficie ovarica normale o cellule di cancro ovarico epiteliale. Discutiamo l'uso di questi modelli per studiare stroma-epiteliali interazioni che si verificano durante lo sviluppo del cancro ovarico.
Tags
Heterotypic Three-dimensional In Vitro ModelingStromal-epithelial InteractionsOvarian Cancer InitiationOvarian Cancer ProgressionEpithelial Ovarian CancersGynecological MalignancySurgical TreatmentsPlatinum-based ChemotherapiesEOC Survival RatesStage I TumorsStage III/IV DiseaseMortality RatesMolecular Genetic BasisBiological BasisEarly Stage Disease DevelopmentMicroenvironment RoleRisk Factors For EOCsNormal Ovary Co-cultureOvarian Surface Epithelial CellsStromal Fibroblast Cells
