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的斑马鱼胚胎神经上皮通透性的测定
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
An Assay for Permeability of the Zebrafish Embryonic Neuroepithelium
DOI:

06:37 min

October 24, 2012

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Chapters

  • 00:05Title
  • 01:05Preparing Injection Needles and Embryos
  • 02:22Injecting Brain Ventricles
  • 03:22Image and Quantification of Dye Movement
  • 04:44Representative Permeability Results of Neuroepithelium
  • 06:18Conclusion

Summary

Automatic Translation

我们描述了现场的整个动物的胚胎斑马鱼脑通透性的定量测量。该技术分析神经管的管腔内保留脑脊液和不同分子量的分子的能力,和它们的运动量化满分心室。此方法是非常有用的用于确定在上皮通透性和成熟发展和疾病过程中的差异。

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