Journal
/
Immunology and Infection
/
饲养和注射<em>烟草天蛾</em>幼虫评估细菌的毒力
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
Rearing and Injection of Manduca sexta Larvae to Assess Bacterial Virulence
Please note that all translations are automatically generated. Click here for the English version.

饲养和注射<em>烟草天蛾</em>幼虫评估细菌的毒力

DOI:
10.3791/4295-v

•

09:04 min

•

December 11, 2012

•

,
1Department of Bacteriology,University of Wisconsin-Madison

Chapters

  • 00:05Title
  • 01:06Insect Egg Sterilization and Rearing
  • 04:06Preparation of Bacteria for Injection
  • 04:44Injection of 4th Instar Larvae
  • 07:46Results: Representative Insect Mortality Assay
  • 08:40Conclusion

Summary

Automatic Translation

Automatic Translation

这里所描述的方法采用直接注射昆虫病原细菌进入血淋巴<em>烟草天蛾</em>昆虫的幼虫。<em> M.天蛾</em是市售和充分研究昆虫。因此，此方法是一种简单的方法来从一个或两个合作伙伴的角度分析宿主细菌的相互作用。

Tags

Manduca SextaTobacco HornwormAgricultural PestSolanaceous PlantsEntomopathogenic BacteriaInsect Immune SystemGenome SequenceModel OrganismHost-microbe InteractionsPathogenesisLarvae InjectionVirulence CharacteristicsBacterial SpeciesMutantsTime To Insect DeathXenorhabdus SpeciesPhotorhabdus SpeciesNematode VectorsInsect Hemolymph

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

唾液，唾液腺，淋巴收集肩突硬蜱蜱

监视分泌细菌毒力因子的组装使用站点特定的交联

生成和多表型高内涵筛选<em>贝氏柯克斯体</em>转座子突变体

施加诱导表达系统研究与细胞内信号细菌毒力因子的干扰

斑马鱼作为一种模型来评估亚硝酸盐的潜在致畸

马达加斯加嘘声蟑螂作为非哺乳动物的替代动物模型来研究毒力、致病机制和药效

吞噬细胞内复制脓肿分枝杆菌毒力标志物的鉴定

线虫模型中气单胞菌感染的毒力及发病机制评价

一种高通量志贺氏菌特异性杀菌试验

评估细菌病原体免疫反应的声吞噬杀灭试验

Read Article