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使用时间的推移显微镜可视化缺氧诱导假死<em> C.线虫</em>胚胎
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JoVE Journal Biology
Use of Time Lapse Microscopy to Visualize Anoxia-induced Suspended Animation in C. elegans Embryos
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使用时间的推移显微镜可视化缺氧诱导假死<em> C.线虫</em>胚胎

DOI:
10.3791/4319-v

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08:49 min

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December 03, 2012

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, ,
1Department of Biological Sciences,University of North Texas

Chapters

  • 00:05Title
  • 02:02Sample Preparation
  • 03:15Slide Preparation and Anoxia Chamber Setup
  • 05:52Microscopy in Anoxia
  • 07:36Representative Embryo Imaging Results
  • 08:10Conclusion

Summary

Automatic Translation

Automatic Translation

这里描述的是一个<em在体内</em>技术图像亚细胞结构在动物中使用的气体流通过在纺丝盘共聚焦显微镜连同microincubation室暴露于缺氧。这种方法非常简单，具有足够的灵活性，以适应各种实验参数和模型系统。

Tags

Time Lapse MicroscopyAnoxia-induced Suspended AnimationC. Elegans EmbryosStress ResistanceGenetic MutantsTransgenic StrainsFusion ProteinsFluorescently Labeled Reporter ProteinsMitosisIn Vivo ImagingSub-cellular StructuresAnoxic StressGas Flow SetupHigh-powered MicroscopeMicroincubation ChamberNitrogen Gas Flow ThroughSpinning Disc Confocal MicroscopeControlled EnvironmentGFP-tagged Gamma TubulinHistoneCell Division ArrestOxygen Deprivation

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