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使用屏幕在车身尺寸规例中的线虫基因介导的RNA干扰取食策略<em> C.线虫</em

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Biology

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JoVE Journal Biology

Using RNA-mediated Interference Feeding Strategy to Screen for Genes Involved in Body Size Regulation in the Nematode C. elegans

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使用屏幕在车身尺寸规例中的线虫基因介导的RNA干扰取食策略<em> C.线虫</em

DOI:

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11:22 min

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February 13, 2013

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Jun Liang, Sheng Xiong³, Cathy Savage-Dunn³

¹Department of Science,Borough of Manhattan Community College, City Universtiy of New York (CUNY), ²Department of Biology,Queens College, The City University of New York (CUNY), ³Biochemistry Program, The Graduate Center,Queens College, The City University of New York (CUNY)

Chapters

00:05Title
01:20Preparing RNAi Feeding Plates Carrying Target Gene Sequence
03:14Culture Worms on the RNAi Feeding Plates
04:58Score Body Size Phenotypes of RNAi Treated Worms
08:46Body Size Regulation by the DBL-1/TGF-β Pathway
11:00Conclusion

Summary

Automatic Translation

我们演示了如何使用供给的RNAi技术敲除目标基因和分体尺寸型<em> C.线虫</em>。这种方法可用于大规模的画面，以找出潜在的基因成分，如在车身尺寸上的DBL-1/TGF-β信号调节。

Tags

RNA-mediated Interference Screening Genes Body Size Regulation Nematode C. Elegans Double-strand RNA Knockdown Target Gene Expression RNAi Vector RNAi Oligos Microinjection Feeding Strategy Body Size Phenotype TGF-β Signaling Components RNAi Hypersensitive Strains

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