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Brug af RNA-medieret Interferens fodringsstrategien at screene for gener involveret i Body Size forordning i Nematode<em> C. elegans</em
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Using RNA-mediated Interference Feeding Strategy to Screen for Genes Involved in Body Size Regulation in the Nematode C. elegans

Brug af RNA-medieret Interferens fodringsstrategien at screene for gener involveret i Body Size forordning i Nematode<em> C. elegans</em

DOI:
10.3791/4373-v

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11:22 min

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February 13, 2013

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, ,
1Department of Science,Borough of Manhattan Community College, City Universtiy of New York (CUNY), 2Department of Biology,Queens College, The City University of New York (CUNY), 3Biochemistry Program, The Graduate Center,Queens College, The City University of New York (CUNY)

Chapters

  • 00:05Title
  • 01:20Preparing RNAi Feeding Plates Carrying Target Gene Sequence
  • 03:14Culture Worms on the RNAi Feeding Plates
  • 04:58Score Body Size Phenotypes of RNAi Treated Worms
  • 08:46Body Size Regulation by the DBL-1/TGF-β Pathway
  • 11:00Conclusion

Summary

Automatic Translation

Automatic Translation

Vi viser, hvordan du bruger RNAi fodring teknik til at vælte målgener og score kropsstørrelse fænotype i<em> C. elegans</em>. Denne metode kunne anvendes til stor skala skærmen for at identificere potentielle genetiske komponenter af interesse, såsom de involveret i kropsstørrelse regulering ved DBL-1/TGF-β signalering.

Tags

RNA-mediated InterferenceScreening GenesBody Size RegulationNematode C. ElegansDouble-strand RNAKnockdown Target Gene ExpressionRNAi VectorRNAi OligosMicroinjectionFeeding StrategyBody Size PhenotypeTGF-β Signaling ComponentsRNAi Hypersensitive Strains

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