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Dual Elektrofysiologiske Opptak av Synaptically-fremkalt Astroglial og Neuronal Responses i akutt hippocampus Slices
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Chapters
- 00:00Title
- 03:58Dissection
- 10:23Dual Recordings of Neuronal fEPSP and Single Astrocytic Whole-cell Response
- 12:07Dual Whole-cell Recordings of Astrocyte and Neuron
- 13:53Representative Results
- 15:45Conclusion
Fremstillingen av akutte hjernen skiver fra isolerte hippocampi samt samtidige elektrofysiologiske innspillinger av astrocytes og nevroner i<em> Stratum radiatum</em> Under stimulering av Schaffer collaterals er beskrevet. Den farmakologiske isolasjon av astroglial kalium og glutamat transporter strømmer blir demonstrert.
Tags
Dual Electrophysiological RecordingsSynaptically-evoked Astroglial ResponsesNeuronal ResponsesAcute Hippocampal SlicesTripartite SynapsesAstrocytesNeuronal Activity ModulationIon ChannelsNeurotransmitter ReceptorsGliotransmittersGlutamate UptakePotassium Spatial BufferingGABA ClearanceSynaptic Activity MonitoringAlterations In Glutamate LevelsAlterations In GABA LevelsAlterations In Extracellular Potassium LevelsAstroglial Uptake ActivityBuffering CapacityPhysiopathological SituationsKnockout MiceHippocampal Slice PreparationStratum Radiatum Astrocytes IdentificationSimultaneous Neuronal And Astroglial RecordingsSynaptically-evoked Astroglial Currents Isolation
