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JoVE Journal Neuroscience
Imaging Receptor Insertion in die Plasmamembran pHluorin-Tag in der Grundschule Cultured Mäuseneuronen
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Imaging Receptor Insertion in die Plasmamembran pHluorin-Tag in der Grundschule Cultured Mäuseneuronen

Article DOI: 10.3791/4450
November 20th, 2012 •
1, 1, 1, 1, 1, 1
1The Jackson Laboratory

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November 20th, 2012

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Durch die Kennzeichnung die extrazellulären Domänen von Membranrezeptoren mit superecliptic pHluorin, und durch Abbilden diese Fusion Rezeptoren in kultivierten Maus Neuronen, können wir direkt visualisieren einzelnen vesikulären Insertionsereignisse der Rezeptoren in der Plasmamembran. Diese Technik wird maßgeblich bei der Aufklärung der molekularen Mechanismen, die Rezeptor-Insertion in die Plasmamembran.

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