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JoVE Journal Neuroscience
Imagiologia pHluorin-tagged Inserção receptor para a membrana plasmática em primários de rato cultivadas Neurons
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Imagiologia pHluorin-tagged Inserção receptor para a membrana plasmática em primários de rato cultivadas Neurons

Article DOI: 10.3791/4450
November 20th, 2012 •
1, 1, 1, 1, 1, 1
1The Jackson Laboratory

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November 20th, 2012

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Ao marcar os domínios extracelulares dos receptores de membrana com pHluorin superecliptic, e por imagiologia estes receptores de fusão em neurónios de rato em cultura, pode-se visualizar directamente os eventos de inserção individuais vesiculares dos receptores na membrana plasmática. Esta técnica será útil para elucidar os mecanismos moleculares que regulam a inserção do receptor para a membrana plasmática.

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