Journal
/
/
成像pHluorin标记插入到质膜受体原代培养的小鼠神经元
JoVE Journal
Neuroscience
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Neuroscience
Imaging pHluorin-tagged Receptor Insertion to the Plasma Membrane in Primary Cultured Mouse Neurons
DOI:

12:58 min

November 20, 2012

, , , , ,

Chapters

  • 00:05Title
  • 01:43Preparing Mouse Glial Culture for Neuronal Culture Conditioning
  • 05:10Neuronal Culture
  • 07:51Transfection
  • 09:41TIRF Imaging
  • 11:12Representative TIRF Images of pHluorin-DRD2 Insertion
  • 12:30Conclusion

Summary

Automatic Translation

通过标记与superecliptic pHluorin膜受体的胞外域的,并通过成像这些融合在培养的小鼠神经元受体,我们可以直接可视化个别水泡插入事件到质膜的受体。这种技术将有助于阐明受体插入到质膜的分子机制。

Related Videos

Read Article