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Analyzing Murine Schwann Cell Development Along Growing Axons
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JoVE Journal Neuroscience
Analyzing Murine Schwann Cell Development Along Growing Axons

Analyzing Murine Schwann Cell Development Along Growing Axons

DOI:
10.3791/50016-v

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09:46 min

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November 21, 2012

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,
1Department of Molecular Embryology, Institute of Anatomy and Cell Biology,University of Freiburg , 2Department of Neuroanatomy,University of Heidelberg, 3FRIAS,University of Freiburg

Chapters

  • 00:05Title
  • 01:27Preparation of Collagen Gels
  • 02:27Dissection of Embryonic Superior Cervical Ganglia
  • 04:34Treatment of Superior Cervical Ganglion Explants
  • 05:55Immunohistochemistry and Quantification of Schwann Cell Migration Distances
  • 07:38Results: Schwann Cell Development Along Growing Axons
  • 09:12Conclusion

Summary

Automatic Translation

Automatic Translation

Here we describe a Schwann cell (SC) migration assay in which SCs are able to develop along extending axons.

Tags

Keywords: Schwann Cell DevelopmentMurineAxon GrowthPeripheral Nervous SystemNeural CrestSuperior Cervical GanglionNerve Growth FactorTime-lapse Imaging

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