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基因改造和重组唾液的腺体器官培养

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Biology

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JoVE Journal Biology

Genetic Modification and Recombination of Salivary Gland Organ Cultures

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基因改造和重组唾液的腺体器官培养

DOI:

10.3791/50060-v

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09:29 min

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January 28, 2013

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Sharon J. Sequeira*¹, Elise M. Gervais*¹, Shayoni Ray, Melinda Larsen

¹Department of Biological Sciences,University at Albany, SUNY

Chapters

00:05Title
01:26Mouse Embryonic Submandibular Gland Harvesting and Microdissection
03:12Separation of Mesenchyme from Submandibular Epithelial Rudiments
04:33Adenoviral Infection of Submandibular Epithelial Rudiments
06:01Recombination of Epithelium and Mesenchyme and Ex vivo Culture
07:02Ex vivo Recombined Gland Post-processing and Imaging
08:37Conclusion

Summary

Automatic Translation

一个基因操纵技术上皮细胞内全<em>体外</em培养的胚胎小鼠颌下腺（冲锋枪）病毒转基因。这种方法利用的先天能力，SMG上皮细胞和间充质，自发重组后的分离和感染的上皮与腺病毒载体的雏形。

Tags

Genetic Modification Recombination Salivary Gland Organ Cultures Branching Morphogenesis Submandibular Gland Epithelial-mesenchymal Interactions Adenoviral Transduction Epithelial Cells Mesenchyme Extracellular Matrix Regenerative Medicine Developmental Biology

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