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Chapters
- 00:05Title
- 01:26Mouse Embryonic Submandibular Gland Harvesting and Microdissection
- 03:12Separation of Mesenchyme from Submandibular Epithelial Rudiments
- 04:33Adenoviral Infection of Submandibular Epithelial Rudiments
- 06:01Recombination of Epithelium and Mesenchyme and Ex vivo Culture
- 07:02Ex vivo Recombined Gland Post-processing and Imaging
- 08:37Conclusion
A technique to genetically manipulate epithelial cells within whole ex vivo cultured embryonic mouse submandibular glands (SMGs) using viral gene transfer is described. This method takes advantage of the innate ability of SMG epithelium and mesenchyme to spontaneously recombine after separation and infection of epithelial rudiments with adenoviral vectors.
