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Genetisk modifikation og Rekombination af spytkirtel organkulturer
JoVE Journal
Biology
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JoVE Journal Biology
Genetic Modification and Recombination of Salivary Gland Organ Cultures
DOI:

09:29 min

January 28, 2013

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Chapters

  • 00:05Title
  • 01:26Mouse Embryonic Submandibular Gland Harvesting and Microdissection
  • 03:12Separation of Mesenchyme from Submandibular Epithelial Rudiments
  • 04:33Adenoviral Infection of Submandibular Epithelial Rudiments
  • 06:01Recombination of Epithelium and Mesenchyme and Ex vivo Culture
  • 07:02Ex vivo Recombined Gland Post-processing and Imaging
  • 08:37Conclusion

Summary

Automatic Translation

En teknik til at genmanipulere epitelceller i hele<em> Ex vivo</em> Dyrkede embryonale muse submandibulære kirtler (SMGS) ved hjælp af viral genoverførsel er beskrevet. Denne fremgangsmåde drager fordel af den medfødte evne SMG epitel og mesenkym spontant at rekombinere efter separation og infektion af epitelceller ansatser med adenovirale vektorer.

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