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Cryosectioning Gjær Communities for å undersøke Fluorescens Patterns
JoVE Journal
Biology
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JoVE Journal Biology
Cryosectioning Yeast Communities for Examining Fluorescence Patterns
DOI:

07:03 min

December 26, 2012

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Chapters

  • 00:05Title
  • 00:58Fixing Yeast Communities
  • 03:40Sectioning Frozen Communities for Fluorescence Imaging
  • 05:15Results: Fluorescence Images of Vertical Cross-sections of Yeast Communities
  • 06:44Conclusion

Summary

Automatic Translation

Vi presenterer en protokoll for frysing og cryosectioning gjær samfunn for å observere interne mønstre av fluorescerende celler. Metoden baserer seg på metanol-innfesting og OCT-innebygging for å bevare den romlige fordelingen av celler uten å inaktivere fluorescerende proteiner i et fellesskap.

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