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鉴定<em>睡美人</em>转座子插入连接器介导的PCR实体瘤

JoVE Journal
Medicine

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JoVE Journal Medicine

Identification of Sleeping Beauty Transposon Insertions in Solid Tumors using Linker-mediated PCR

Please note that all translations are automatically generated. Click here for the English version.

鉴定<em>睡美人</em>转座子插入连接器介导的PCR实体瘤

DOI:

10.3791/50156-v

•

10:34 min

•

February 01, 2013

•

Callie L. Janik², Timothy K. Starr²

¹Department of Obstetrics, Gynecology & Women’s Health, Masonic Cancer Center,University of Minnesota, Minneapolis, ²Department of Genetics, Cell Biology & Development, Center for Genome Engineering,University of Minnesota, Minneapolis

Chapters

00:05Title
01:00Tumor Collection and DNA Isolation
02:16Digesting and Ligating Linkers to the DNA
05:51Removing Concatamer Transposons Using a Second Digestion
06:54Amplifying the Tumor DNA
09:19PCR and Sequencing Results
10:08Conclusion

Summary

Automatic Translation

鉴定未知的驱动程序致癌作用的一种方法，用一种公正的方法。该方法使用<em>睡美人</em>转座子作为随机诱变剂，向特定的组织。肿瘤的形成转座子插入驱动的新型癌基因和肿瘤抑制基因的基因组作图

Tags

Sleeping Beauty Transposon Forward Genetic Screen Linker-mediated PCR Tumor Genetics Cancer Genome Genetic Drivers Passenger Mutations Genetic Heterogeneity Cre Recombinase Transposase Common Insertion Sites

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