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鉴定<em>睡美人</em>转座子插入连接器介导的PCR实体瘤
JoVE Journal
Medicine
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JoVE Journal Medicine
Identification of Sleeping Beauty Transposon Insertions in Solid Tumors using Linker-mediated PCR
DOI:

10:34 min

February 01, 2013

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Chapters

  • 00:05Title
  • 01:00Tumor Collection and DNA Isolation
  • 02:16Digesting and Ligating Linkers to the DNA
  • 05:51Removing Concatamer Transposons Using a Second Digestion
  • 06:54Amplifying the Tumor DNA
  • 09:19PCR and Sequencing Results
  • 10:08Conclusion

Summary

Automatic Translation

鉴定未知的驱动程序致癌作用的一种方法,用一种公正的方法。该方法使用<em>睡美人</em>转座子作为随机诱变剂,向特定的组织。肿瘤的形成转座子插入驱动的新型癌基因和肿瘤抑制基因的基因组作图

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