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Medicine
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延时成像的主要癌前乳腺上皮细胞来源于遗传工程小鼠乳腺癌模型
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JoVE Journal
Medicine
Time-lapse Imaging of Primary Preneoplastic Mammary Epithelial Cells Derived from Genetically Engineered Mouse Models of Breast Cancer
Please note that all translations are automatically generated.
Click here for the English version.
延时成像的主要癌前乳腺上皮细胞来源于遗传工程小鼠乳腺癌模型
DOI:
10.3791/50198-v
•
11:47 min
•
February 08, 2013
•
Rebecca E. Nakles
,
Sarah L. Millman
,
M. Carla Cabrera
,
Peter Johnson
2
,
Susette Mueller
2
,
Philipp S. Hoppe
,
Timm Schroeder
,
Priscilla A. Furth
2,4,5
1
Department of Oncology
,
Georgetown University
,
2
Lombardi Comprehensive Cancer Center
,
Georgetown University
,
3
Stem Cell Dynamics
,
Helmholtz Zentrum München – German Research Center for Environmental Health
,
4
Department of Medicine
,
Georgetown University
,
5
Department of Nanobiomedical Science and WCU Research Center of Nanobiomedical Science
,
Dankook University
Chapters
00:05
Title
01:48
Generation of Primary Mammary Epithelial Cell Cultures
03:39
Live-cell Imaging
05:56
Assessing Cellular Event from Time-lapse Images
07:50
Making Single Cell Fate Maps with Timm’s Tracking Tool (TTT)
09:59
Representative Results: Colony Formation in BRCA1 Disrupted Cells
11:12
Conclusion
Summary
Automatic Translation
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Automatic Translation
时间延时成像是用来评估初级癌前来自乳腺癌风险遗传工程小鼠模型,以确定是否有特定的行为参数之间的相关性和不同的遗传病变的乳腺上皮细胞的行为。
Tags
Time-lapse Imaging
Primary Preneoplastic Mammary Epithelial Cells
Genetically Engineered Mouse Models
Breast Cancer
Cell Division
Apoptosis
Colony Formation
Cell Morphology
Single-cell Tracking
Cell Fate Maps
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