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通过微接触印刷的定向功能性心肌组织的产生
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Generation of Aligned Functional Myocardial Tissue Through Microcontact Printing
DOI:

11:09 min

March 19, 2013

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Chapters

  • 00:05Title
  • 01:19Microcontact Printing of Fibronectin onto PDMS Substrates
  • 02:46Maintenance of Double Transgenic Embryonic Stem Cell Lines
  • 04:37In vitro Differentiation of Double Transgenic Embryonic Stem Cell Lines and Isolation and Seeding of Cardiac Progenitors
  • 08:14Results: Highly Purified Populations of Embryonic Stem Cell Derived Cardiac Progenitors
  • 10:51Conclusion

Summary

Automatic Translation

对齐心肌组织的产生是一个关键的要求,适应干细胞生物学的最新进展,临床上有用的目的。在这里,我们描述了一个微接触印刷方法的精确控制细胞的形状和功能。使用高度纯化群体中的胚胎干细胞源性心肌祖细胞,然后产生各向异性功能的心肌组织。

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