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Oprettelse Lim og Opløseligt Gradienter for Imaging Cell Migration med Fluorescence Microscopy
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Chapters
- 00:05Title
- 02:17Passivation of Glass Coverslips with PLL-PEG-biotin
- 04:04Fabrication of Stamps for Microcontact Printing
- 04:38Patterning Streptavidin or Adhesive Cues onto Passivated Glass Coverslips
- 06:13Creating Adhesive Gradients onto Streptavidin-pattern Surfaces
- 07:47Loading of Cells with a Soluble Gradient into the Microfluidics Device for Live Cell Imaging
- 09:08Results: Cell Migration in Response to Adhesive and Soluble Gradients
- 12:16Conclusion
En fremgangsmåde til samling af lim og opløselige gradienter i en mikroskopi kammer til levende celler migrationsundersøgelser er beskrevet. Den konstruerede miljø kombinerer antifouling overflader og klæbende spor med løsning gradienter og derfor tillader en at bestemme den relative betydning af vejledning cues.
