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JoVE Journal Bioengineering
Oprettelse Lim og Opløseligt Gradienter for Imaging Cell Migration med Fluorescence Microscopy
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Oprettelse Lim og Opløseligt Gradienter for Imaging Cell Migration med Fluorescence Microscopy

Article DOI: 10.3791/50310-v • 13:10 min • April 4th, 2013
April 4th, 2013 •
1, 1, 2, 1, 1, 2, 1, 1
1Centre for Vascular Research and Australian Centre for Nanomedicine, The University of New South Wales, 2School of Chemistry and Australian Centre for Nanomedicine, The University of New South Wales

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En fremgangsmåde til samling af lim og opløselige gradienter i en mikroskopi kammer til levende celler migrationsundersøgelser er beskrevet. Den konstruerede miljø kombinerer antifouling overflader og klæbende spor med løsning gradienter og derfor tillader en at bestemme den relative betydning af vejledning cues.

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Bioengineering Mikrobiologi Cellular Biology biokemi molekylærbiologi biofysik Cell migration live cell imaging opløselige og vedhængende gradienter microcontact udskrivning dip pen litografi MicroFluidics RGD PEG biotin streptavidin kemotaksi kemoattraktant imaging
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