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GST-他的净化:一个两步亲和纯化协议屈服全长纯化的蛋白质
JoVE Journal
Biology
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JoVE Journal Biology
GST-His purification: A Two-step Affinity Purification Protocol Yielding Full-length Purified Proteins
DOI:

11:22 min

October 29, 2013

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Chapters

  • 00:05Title
  • 01:49Recombinant Protein Expression and Preparation of Soluble Cell Lysate
  • 03:17GST Affinity Purification
  • 05:14TALON Metal Affinity Purification
  • 07:00Results: Analysis of GST-His Purification
  • 10:21Conclusion

Summary

Automatic Translation

在本协议中,我们展示了一个高效和具有成本效益的小型蛋白质纯化方法,它允许重组蛋白的纯化结合独特的可裂解GST标签和一个小His标签。

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