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GST-他的净化：一个两步亲和纯化协议屈服全长纯化的蛋白质
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GST-His purification: A Two-step Affinity Purification Protocol Yielding Full-length Purified Proteins
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GST-他的净化：一个两步亲和纯化协议屈服全长纯化的蛋白质

DOI:
10.3791/50320-v

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11:22 min

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October 29, 2013

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, , , , ,
1Genome Stability Laboratory, Oncology Axis,Hôtel-Dieu de Québec

Chapters

  • 00:05Title
  • 01:49Recombinant Protein Expression and Preparation of Soluble Cell Lysate
  • 03:17GST Affinity Purification
  • 05:14TALON Metal Affinity Purification
  • 07:00Results: Analysis of GST-His Purification
  • 10:21Conclusion

Summary

Automatic Translation

Automatic Translation

在本协议中，我们展示了一个高效和具有成本效益的小型蛋白质纯化方法，它允许重组蛋白的纯化结合独特的可裂解GST标签和一个小His标签。

Tags

GST-His PurificationAffinity PurificationProtein Purification ProtocolRecombinant ProteinsGlutathione Sepharose Tag (GST)10xHis TagBaculovirus ExpressionSf9 CellsPreScission EnzymeFull-length ProteinsHeat Shock Protein ImpuritiesNuclease Treatment

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