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Chapters
- 00:05Title
- 01:49Recombinant Protein Expression and Preparation of Soluble Cell Lysate
- 03:17GST Affinity Purification
- 05:14TALON Metal Affinity Purification
- 07:00Results: Analysis of GST-His Purification
- 10:21Conclusion
Tags
GST-His PurificationTwo-step Affinity Purification ProtocolFull-length Purified ProteinsProtein PurificationHigh ConcentrationsBiochemical CharacterizationEnzymology AssaysEfficiencySimplicityCost EffectivenessN-terminal Glutathione Sepharose Tag (GST)C-terminal 10xHis TagRecombinant ProteinsBaculovirusesSf9 Infected CellsProtein ExpressionPreScission EnzymeMaximum PurityCleaved GST RemovalHis-Tag Affinity PurificationDegraded Proteins RemovalFull-length Proteins EnrichmentInstrumental SetupFPLCMgCl2 WashesATP WashesHeat Shock Protein Impurities Removal
