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In vivo Neuronal Calcium Imaging in C. elegans
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
In vivo Neuronal Calcium Imaging in C. elegans
DOI:

11:06 min

April 10, 2013

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Chapters

  • 00:05Title
  • 01:43Optical Setup
  • 04:05Sample Preparation and Data Acquisition
  • 05:33Analysis and Problem Solving Data
  • 07:36Representative Calcium Imaging Results
  • 10:23Conclusion

Summary

Automatic Translation

With its small transparent body, well-documented neuroanatomy and a host of amenable genetic techniques and reagents, C. elegans makes an ideal model organism for in vivo neuronal imaging using relatively simple, low-cost techniques. Here we describe single neuron imaging within intact adult animals using genetically encoded fluorescent calcium indicators.

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