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原代培养大鼠内髓集合管细胞
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JoVE Journal Biology
Culturing Primary Rat Inner Medullary Collecting Duct Cells
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原代培养大鼠内髓集合管细胞

DOI:
10.3791/50366-v

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06:11 min

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June 21, 2013

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, , , , , ,
1Anchored Signalling,Max-Delbrück-Center for Molecular Medicine, 2Leibniz Institute for Molecular Pharmacology (FMP), 3Charité University Medicine Berlin

Chapters

  • 00:05Title
  • 01:24Preparation
  • 02:01Cultivation of Primary Rat Inner Medullary Collecting Duct (IMCD) Cells
  • 05:09Results: IMCD Cell Expression
  • 05:39Conclusion

Summary

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Automatic Translation

精氨酸加压素（AVP）控制体内水分的动态平衡微调肾主细胞，通过促进水的重吸收。在这里，我们提出了一个协议的初级大鼠内髓集合管细胞AVP-介导的水的重吸收的分子机制的阐明适合种植。

Tags

Keywords: Primary Rat Inner Medullary Collecting Duct CellsArginine-vasopressinV2 ReceptorAquaporin-2CAMPProtein Kinase AWater ReabsorptionDiabetes InsipidusHeart FailureCell Culture Protocol

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