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原代培养大鼠内髓集合管细胞
JoVE Journal
Biology
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JoVE Journal
Biology
Culturing Primary Rat Inner Medullary Collecting Duct Cells
Please note that all translations are automatically generated.
Click here for the English version.
原代培养大鼠内髓集合管细胞
DOI:
10.3791/50366-v
•
06:11 min
•
June 21, 2013
•
Dörte Faust
,
Andrea Geelhaar
,
Beate Eisermann
,
Jenny Eichhorst
,
Burkhard Wiesner
,
Walter Rosenthal
3
,
Enno Klussmann
1
Anchored Signalling
,
Max-Delbrück-Center for Molecular Medicine
,
2
Leibniz Institute for Molecular Pharmacology (FMP)
,
3
Charité University Medicine Berlin
Chapters
00:05
Title
01:24
Preparation
02:01
Cultivation of Primary Rat Inner Medullary Collecting Duct (IMCD) Cells
05:09
Results: IMCD Cell Expression
05:39
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
精氨酸加压素(AVP)控制体内水分的动态平衡微调肾主细胞,通过促进水的重吸收。在这里,我们提出了一个协议的初级大鼠内髓集合管细胞AVP-介导的水的重吸收的分子机制的阐明适合种植。
Tags
Primary Rat Inner Medullary Collecting Duct Cells
Arginine-vasopressin
V2 Receptor
Aquaporin-2
CAMP
Protein Kinase A
Water Reabsorption
Diabetes Insipidus
Heart Failure
Cell Culture Protocol
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