/

/

多光谱定量分析继荧光组织移植的细胞起源，类型和相互作用的可视化

JoVE Journal
Medicine

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Medicine

Quantitative Multispectral Analysis Following Fluorescent Tissue Transplant for Visualization of Cell Origins, Types, and Interactions

Please note that all translations are automatically generated. Click here for the English version.

多光谱定量分析继荧光组织移植的细胞起源，类型和相互作用的可视化

DOI:

10.3791/50385-v

•

11:27 min

•

September 22, 2013

•

Erika L. Spaeth, Christopher M. Booth, Frank C. Marini²

¹Department of Leukemia,MD Anderson Cancer Center, ²Wake Forest Baptist Medical Center,Institute for Regenerative Medicine

Chapters

00:05Title
02:06Syngeneic Murine Bone Marrow Transplantation (BMT)
03:11Tumor Engraftment
04:01Multi-parameter Immunofluorescent Staining
06:13Multispectral Imaging
07:10Quantitative Analysis of Multispectral Images
08:00Results
10:32Conclusion

Summary

Automatic Translation

复合组织群众，从机关到肿瘤，是由各种细胞成分的。我们阐明了利用多标记的荧光转基因小鼠与多参数免疫荧光染色结合组织随后光谱分离破译细胞起源以及基于蛋白表达细胞特征的细胞内表型的贡献。

Tags

Multispectral Analysis Cell Origins Cell Types Cell Interactions Tumor Development Bone Marrow Tumor Stroma GFP RFP MIMicc Multiplexed Cellular Compositions Fluoroprobes Immunohistochemistry Cell Lineages Cell Differentiation Tumor Stromal Populations

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

定量可视化和皮肤癌症使用动态热成像检测

肺转移瘤在小鼠体内的组合在单细胞分辨率的可视化改进<em>在原位</em灌注肺组织和X-gal染色<em> lacZ的</em>标记的肿瘤细胞

验尸报告组织心肌细胞核分离

在疾病的染色质蛋白质组的定量分析

小鼠异位心脏移植技术

人类癫痫术后皮层组织的多电极阵列录音

小鼠肾脏移植技术

静脉介入型号：研究旁路移植通畅一个合适的模型

通过轻型显微镜对小鼠急性心肌炎模型中白细胞浸润的定量可视化

球囊损伤诱发小鼠腹主动脉 Myointimal 增生的研究

Read Article