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Immunology and Infection
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比较方法来表征景观的宿主 - 病原体蛋白 - 蛋白相互作用
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Immunology and Infection
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JoVE Journal
Immunology and Infection
A Comparative Approach to Characterize the Landscape of Host-Pathogen Protein-Protein Interactions
Please note that all translations are automatically generated.
Click here for the English version.
比较方法来表征景观的宿主 - 病原体蛋白 - 蛋白相互作用
DOI:
10.3791/50404-v
•
13:56 min
•
July 18, 2013
•
Mandy Muller*
1,2
,
Patricia Cassonnet*
1
,
Michel Favre
,
Yves Jacob
3
,
Caroline Demeret
1
Unité de Génétique, Papillomavirus et Cancer Humain (GPCH)
,
Institut Pasteur
,
2
Cellule Pasteur
,
Université Sorbonne Paris Cité
,
3
Center for Cancer Systems Biology (CCSB), Harvard Medical School, Department of Cancer Biology
,
Dana Farber Cancer Institute
Chapters
00:05
Title
02:02
Yeast Two-hybrid Screenings
05:21
Screening of HIS3 Positive Clones by PCR and Sequencing
07:00
Cell Transfection
08:26
Cell Lysis and Gaussia Luciferase Dosage
09:23
Measurement of Firefly Activity and Normalized Luminescence Ratio (NLR) Calculations
10:29
Results: Characterization of Virus-host Protein-protein Interactions for the E2, E6 and E7 Early Proteins of Human Papillomaviruses
13:05
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本文重点介绍高信心之间的相互作用数据库宿主与病原体的蛋白质结合使用两个正交的方法识别:酵母双杂交,然后通过高通量相互作用的检测在哺乳动物细胞中名为HT-GPCA。
Tags
Protein-protein Interactions
Host-pathogen Interactions
Comparative Interactomics
Yeast Two-hybrid
Gaussia Luciferase Complementation Assay
High-throughput Screening
Pathogen Strain Variants
Protein Interaction Networks
Article
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