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Journal
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Immunology and Infection
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腺病毒转导朴素的CD4 T细胞调节性T细胞分化研究
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Immunology and Infection
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Immunology and Infection
Adenoviral Transduction of Naive CD4 T Cells to Study Treg Differentiation
Please note that all translations are automatically generated.
Click here for the English version.
腺病毒转导朴素的CD4 T细胞调节性T细胞分化研究
DOI:
10.3791/50455-v
•
15:33 min
•
August 13, 2013
•
Sebastian C. Warth
,
Vigo Heissmeyer
1
Institute for Molecular Immunology
,
Helmholtz Zentrum München
Chapters
00:05
Title
02:03
Cloning of a Gene of Interest into an Entry Vector
03:16
Generation of the Primary Virus Lysate
05:47
Virus Amplification
07:34
T Cell Infection
09:01
T Cell Activation and Polarization
10:01
T Cell Fixation Staining for Flow Cytometry
11:51
Infection Does Not Interfere with T Cell Activation
14:42
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
腺病毒基因转移到幼稚的CD4 T细胞与柯萨奇腺病毒受体的转基因表达,使调节性T细胞分化的分子分析<em>在体外</em>。
Tags
Adenoviral Transduction
Naive CD4 T Cells
Regulatory T Cells
Treg Differentiation
Ectopic Gene Expression
Coxsackie Adenovirus Receptor
TCR Activation
TGF-beta
IL-2
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