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Immunology and Infection
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ナイーブCD4のアデノウイルス形質導入Tregの分化を研究するためにT細胞
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Immunology and Infection
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JoVE Journal
Immunology and Infection
Adenoviral Transduction of Naive CD4 T Cells to Study Treg Differentiation
Please note that all translations are automatically generated.
Click here for the English version.
ナイーブCD4のアデノウイルス形質導入Tregの分化を研究するためにT細胞
DOI:
10.3791/50455-v
•
15:33 min
•
August 13, 2013
•
Sebastian C. Warth
,
Vigo Heissmeyer
1
Institute for Molecular Immunology
,
Helmholtz Zentrum München
Chapters
00:05
Title
02:03
Cloning of a Gene of Interest into an Entry Vector
03:16
Generation of the Primary Virus Lysate
05:47
Virus Amplification
07:34
T Cell Infection
09:01
T Cell Activation and Polarization
10:01
T Cell Fixation Staining for Flow Cytometry
11:51
Infection Does Not Interfere with T Cell Activation
14:42
Conclusion
Summary
Automatic Translation
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Automatic Translation
ナイーブCD4コクサッキーアデノウイルス受容体のトランスジェニック発現とT細胞へのアデノウイルス遺伝子導入は、制御性T細胞分化の分子解析を可能に<em> in vitroで</em>。
Tags
Adenoviral Transduction
Naive CD4 T Cells
Regulatory T Cells
Treg Differentiation
Ectopic Gene Expression
Coxsackie Adenovirus Receptor
TCR Activation
TGF-beta
IL-2
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