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量化衰老癌症细胞的敏感方法
JoVE Journal
Medicine
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JoVE Journal Medicine
A Sensitive Method to Quantify Senescent Cancer Cells
DOI:

09:18 min

August 02, 2013

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Chapters

  • 00:05Title
  • 01:52Preparation of C12FDG, Bafilomycin A1, and Cell Culture
  • 03:22Induction of Senescence and Bafilomycin A1 Treatment
  • 04:38C12FDG Staining
  • 05:29Flow Cytometry Analysis
  • 07:20Representative Assay Results
  • 08:39Conclusion

Summary

Automatic Translation

无论是衰老阻止或促进肿瘤的发生仍存在争议。由于化学治疗药物可以诱导癌细胞衰老,衰老研究提出的新疗法是必不可少的。然而,标准的和广泛使用的β-半乳糖苷酶测定的主要缺点。在这里我们提出一种快速,灵敏的基于流式细胞仪检测,以量化衰老。

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