Journal
/
Bioengineering
/
使用原子力显微镜测量活细胞的力学性能
JoVE Journal
Bioengineering
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Bioengineering
Measuring the Mechanical Properties of Living Cells Using Atomic Force Microscopy
Please note that all translations are automatically generated. Click here for the English version.

使用原子力显微镜测量活细胞的力学性能

DOI:
10.3791/50497-v

•

08:41 min

•

June 27, 2013

•

, , ,
1Department of Physics,Worcester Polytechnic Institute, 2Department of Chemical Engineering,Worcester Polytechnic Institute

Chapters

  • 00:05Title
  • 01:10Calibrate the Spring Constant of Cantilever
  • 02:56AFM/Cell Culture Preparation
  • 04:00Collecting Force Curves of Cell Indentation
  • 05:36Results: AFM Measurement of Cell Stiffness
  • 06:59Results: AFM Measurement of Cell Stiffness
  • 08:19Conclusion

Summary

Automatic Translation

Automatic Translation

本文演示了一个协议，通过使用原子力显微镜（AFM）的显微表征活细胞的机械性能。

Tags

Atomic Force MicroscopyCell StiffnessExtracellular MatrixHertz ModelMicro-indentationCell MechanicsCell Physiology

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

原子力显微镜成像的细菌固定化

重组DNA和蛋白质复合物，用原子力显微镜的可视化

量化的内皮细胞糖复合物的力学性能，用原子力显微镜

新方法本地化记者荧光珠近细胞培养表面的牵引力显微镜

细菌表面电位测量用开尔文探针力显微镜

原子力显微镜成像和支持的脂质双层力谱

在生物膜的力学性能原位制图粒子追踪Microrheology

利用合成生物学工程师活细胞接口，具有可编程材料

测量海洋海绵针力学性能的毫米尺度弯曲试验系统

荧光技术结合测定活细胞中的力敏感蛋白动力学

Read Article