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Medir as propriedades mecânicas das células vivas Usando Microscopia de Força Atômica
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Measuring the Mechanical Properties of Living Cells Using Atomic Force Microscopy
DOI:

08:41 min

June 27, 2013

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Chapters

  • 00:05Title
  • 01:10Calibrate the Spring Constant of Cantilever
  • 02:56AFM/Cell Culture Preparation
  • 04:00Collecting Force Curves of Cell Indentation
  • 05:36Results: AFM Measurement of Cell Stiffness
  • 06:59Results: AFM Measurement of Cell Stiffness
  • 08:19Conclusion

Summary

Automatic Translation

Este documento revela um protocolo para caracterizar as propriedades mecânicas de células vivas por meio de microindentação utilizando um microscópio de força atómica (AFM).

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