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Kraftmaalingen Under Sammentrækning at vurdere muskelfunktionen hos zebrafisk Larver
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Biology
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JoVE Journal Biology
Force Measurement During Contraction to Assess Muscle Function in Zebrafish Larvae

Kraftmaalingen Under Sammentrækning at vurdere muskelfunktionen hos zebrafisk Larver

DOI:
10.3791/50539-v

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08:25 min

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July 23, 2013

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, , ,
1Department of Biomedical Engineering,University of Michigan , 2Department of Surgery, Section of Plastic Surgery,University of Michigan , 3Departments of Pediatrics and Neurology,University of Michigan , 4Department of Molecular and Integrative Physiology,University of Michigan

Chapters

  • 00:05Title
  • 01:12Preparing Suture Loops
  • 01:50Preparing the Experimental Chamber and Positioning the Larva
  • 04:11Recording Force During a Maximal Twitch Contraction
  • 06:20Measuring Musculature Dimensions with Larva at Optimal Length
  • 07:00Results: Peak Force During Maximal Twitch
  • 07:56Conclusion

Summary

Automatic Translation

Automatic Translation

Kraftmålinger kan bruges til at påvise ændringer i musklernes funktion på grund af udviklingen, skade, sygdom, behandling eller kemisk toksicitet. I denne video viser vi en metode til at måle kraft i løbet af en maksimal sammentrækning af zebrafisk larver trunk muskler.

Tags

Force MeasurementMuscle FunctionZebrafish LarvaeMuscle ContractionIsometric TwitchForce TransducerLength ControllerElectric Field StimulationMuscle DevelopmentMuscle DiseaseMuscle Toxicity

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