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提高蛋白质结晶的成功率随机Microseed矩阵筛选
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Improving the Success Rate of Protein Crystallization by Random Microseed Matrix Screening
Please note that all translations are automatically generated.
Click here for the English version.
提高蛋白质结晶的成功率随机Microseed矩阵筛选
DOI:
10.3791/50548-v
•
12:24 min
•
August 31, 2013
•
Marisa Till
,
Alice Robson
,
Matthew J. Byrne
,
Asha V. Nair
,
Stefan A. Kolek
,
Patrick D. Shaw Stewart
,
Paul R. Race
1
School of Biochemistry
,
University of Bristol
,
2
Douglas Instruments
Chapters
00:05
Title
01:58
Preparation of the Seed Stock
06:09
Establishment of Crystallization Trays
08:45
Inspection of Crystallization Trays
09:40
Representative rMMS Experiment
11:36
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里,我们描述了随机microseed矩阵筛选的一般方法。这种技术被示为显著增加的蛋白质结晶筛选实验的成功率,减少需要优化,并提供晶体的数据收集和配体浸泡实验的可靠供应。
Tags
Protein Crystallization
Random Microseed Matrix Screening
RMMS
Vapor Diffusion
Sitting Drop
Hanging Drop
96-well
24-well
Liquid Handling Robotics
Protein Phase Diagram
Metastable Zone
Crystal Quality
Crystal Supply
Soaking Experiments
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