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Forbedre suksessrate på Protein Krystallisering av Random Microseed Matrix Screening
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JoVE Journal Biology
Improving the Success Rate of Protein Crystallization by Random Microseed Matrix Screening

Forbedre suksessrate på Protein Krystallisering av Random Microseed Matrix Screening

DOI:
10.3791/50548-v

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12:24 min

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August 31, 2013

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, , , , , ,
1School of Biochemistry,University of Bristol, 2Douglas Instruments

Chapters

  • 00:05Title
  • 01:58Preparation of the Seed Stock
  • 06:09Establishment of Crystallization Trays
  • 08:45Inspection of Crystallization Trays
  • 09:40Representative rMMS Experiment
  • 11:36Conclusion

Summary

Automatic Translation

Automatic Translation

Her beskriver vi en generell metode for tilfeldig microseed matrise screening. Denne teknikken er vist å signifikant øke suksessraten av protein krystallisering screening eksperimenter, redusere behovet for optimalisering, og gir en pålitelig forsyning av krystaller for datainnsamling og ligand-avslapnings eksperimenter.

Tags

Protein CrystallizationRandom Microseed Matrix ScreeningRMMSVapor DiffusionSitting DropHanging Drop96-well24-wellLiquid Handling RoboticsProtein Phase DiagramMetastable ZoneCrystal QualityCrystal SupplySoaking Experiments

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