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基因编码的分子探针来研究G蛋白偶联受体
JoVE Journal
Biology
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JoVE Journal Biology
Genetically-encoded Molecular Probes to Study G Protein-coupled Receptors
DOI:

16:16 min

September 13, 2013

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Chapters

  • 00:05Title
  • 02:23Site-specific Genetic Incorporation of Unnatural Amino Acids into GPCRs
  • 03:15Mapping a Ligand Binding Site Using Targeted Photocrosslinking
  • 07:12Targeted Epitope Tagging and Cell Surface Enzyme-linked Immunosorbent Assay (ELISA)
  • 10:23Bioorthogonal Fluorescent Labeling of a GPCR
  • 12:10Results: Versatile Applications of Site-specific azF Incorporation into GPCRs

Summary

Automatic Translation

在GPCR的各种目标的位置,我们基因编码的非天然氨基酸,对 - 叠氮基-L-苯丙氨酸,并显示在叠氮基团中的不同的应用程序的通用性。这些包括有针对性的光交联技术,以确定在一个G蛋白偶联受体的配体结合口袋的残基,和位点特异性生物正交的GPCR与肽表位标签或荧光探针的修饰。

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