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Journal
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Bioengineering
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氧微环境中的单个胰岛水平的定量和临时控制
JoVE Journal
Bioengineering
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JoVE Journal
Bioengineering
Quantitative and Temporal Control of Oxygen Microenvironment at the Single Islet Level
Please note that all translations are automatically generated.
Click here for the English version.
氧微环境中的单个胰岛水平的定量和临时控制
DOI:
10.3791/50616-v
•
11:49 min
•
November 17, 2013
•
Joe Fu-Jiou Lo
,
Yong Wang
3
,
Zidong Li
,
Zhengtuo Zhao
,
Di Hu
,
David T. Eddington
,
Jose Oberholzer
3
1
Department of Mechanical Engineering
,
University of Michigan-Dearborn
,
2
Department of Surgery/Transplant
,
University of Illinois at Chicago
,
3
Department of Bioengineering
,
University of Illinois at Chicago
Chapters
00:05
Title
01:59
Microfluidic Platform Fabrication
05:17
Microdispenser Setup
06:53
Isolation and Staining of Mouse Islets
08:25
Simultaneous Oxygen and Glucose Stimulations
09:19
Results: Intermittent Hypoxia Preconditioning Improves Islet Responses to Hypoxia
10:46
Conclusion
Summary
Automatic Translation
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Automatic Translation
微流控氧赋予的不仅仅是方便和速度超过低氧室的生物实验。通过扩散穿过膜实现的,特别是当,微氧可以在微尺度级同时提供液相和气相的调制。这项技术使动态多参数实验的关键学习islet病理生理学。
Tags
Oxygen Microenvironment
Islet
Microfluidic
Hypoxia
Glucose Stimulus-secretion Coupling
Calcium Dynamics
KATP Channel
Preconditioning
Real-time Monitoring
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