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JoVE Journal
Immunology and Infection
Author Produced

This content is Open Access.

Optimized Protocols for Mycobacterium leprae Strain Management
 

Optimized Protocols for Mycobacterium leprae Strain Management: Frozen Stock Preservation and Maintenance in Athymic Nude Mice

Article doi: 10.3791/50620
March 23rd, 2014

Summary March 23rd, 2014

Mycobacterium leprae, the causative agent of leprosy, does not grow in vitro. We describe an easy to follow protocol to prepare a bacillary suspension to ensure the maintenance of large quantities of M. leprae for a variety of applications. Protocols for propagation by mouse footpad inoculation, evaluation of viability, freezing and thawing bacillary stock are described in detail.

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