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Biosensores radiométricos que miden el estado redox mitocondrial y ATP en las células vivas de levadura
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Ratiometric Biosensors that Measure Mitochondrial Redox State and ATP in Living Yeast Cells
DOI:

12:22 min

July 22, 2013

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Chapters

  • 00:05Title
  • 02:48Growth of Cells and Preparation for Imaging
  • 03:29Mito-roGFP1 Imaging
  • 04:33MitGO-ATeam2 Imaging
  • 06:09ImageJ Analysis
  • 08:40Measuring Mitochondrial Redox State and ATP with Mito-roGFP1 and MitGO-ATeam2
  • 11:22Conclusion

Summary

Automatic Translation

Se describe el uso de dos, biosensores ratiometric codificados genéticamente, que se basan en la GFP, para vigilar los niveles de ATP estado redox mitocondrial y en la resolución subcelular en células de levadura vivas.

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