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成比例的生物传感器测量活酵母细胞中的线粒体氧化还原状态和ATP
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JoVE Journal
Bioengineering
Ratiometric Biosensors that Measure Mitochondrial Redox State and ATP in Living Yeast Cells
Please note that all translations are automatically generated.
Click here for the English version.
成比例的生物传感器测量活酵母细胞中的线粒体氧化还原状态和ATP
DOI:
10.3791/50633-v
•
12:22 min
•
July 22, 2013
•
Jason D. Vevea*
1
,
Dana M. Alessi Wolken*
1
,
Theresa C. Swayne
,
Adam B. White
,
Liza A. Pon
1
Department of Pathology and Cell Biology
,
Columbia University
,
2
Herbert Irving Comprehensive Cancer Center
,
Columbia University
Chapters
00:05
Title
02:48
Growth of Cells and Preparation for Imaging
03:29
Mito-roGFP1 Imaging
04:33
MitGO-ATeam2 Imaging
06:09
ImageJ Analysis
08:40
Measuring Mitochondrial Redox State and ATP with Mito-roGFP1 and MitGO-ATeam2
11:22
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们描述了使用两个比例,基因编码生物传感器的,这是基于GFP,监测线粒体的氧化还原状态和细胞内ATP水平分辨率在活酵母细胞。
Tags
Mitochondrial Redox State
Mitochondrial ATP
Ratiometric Biosensors
RoGFP
MitGO-ATeam
FRET
Yeast Cells
Energy Metabolism
Calcium Homeostasis
Cellular Lifespan
Programmed Cell Death
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