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1Center for Environmental Medicine, Asthma, and Lung Biology,The University of North Carolina at Chapel Hill, 2Department of Pediatrics,The University of North Carolina at Chapel Hill, 3Pulmonary Diseases and Critical Care,The University of North Carolina at Chapel Hill, 4Curriculum in Toxicology,The University of North Carolina at Chapel Hill
Chapters
- 00:05Title
- 02:05Obtaining Biopsy of Human NECs
- 03:37Seeding NECs on Plastic
- 04:41Expanding the Cells in Flasks
- 06:00Seeding Cells on Tissue Culture Inserts
- 07:53Establishing Air Liquid Interface (ALI)
- 08:35ALI Culture of NECs Produces a Differentiated Epithelium
- 09:43Conclusion
Nasale epitelceller, opnået gennem overfladisk skrabe biopsi af frivillige, der er udvidet og overført til vævsdyrkningssystemer skær. Efter at have nået konfluens, dyrkes celler på luft væske-grænsefladen, hvilket gav kulturer af cilierede og ikke-cilierede celler. Differentierede nasale epitel cellekulturer give levedygtige eksperimentelle modeller til at studere det respiratoriske slimhinde.
