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Journal
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Bioengineering
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生产尺寸控制肿瘤细胞球体采用微孔板大数
JoVE Journal
Bioengineering
This content is Free Access.
JoVE Journal
Bioengineering
Production of Large Numbers of Size-controlled Tumor Spheroids Using Microwell Plates
Please note that all translations are automatically generated.
Click here for the English version.
生产尺寸控制肿瘤细胞球体采用微孔板大数
DOI:
10.3791/50665-v
•
10:44 min
•
November 18, 2013
•
Golsa Razian
,
Yang Yu
,
Mark Ungrin
1
Department of Comparative Biology & Experimental Medicine, Faculty of Veterinary Medicine
,
University of Calgary
Chapters
00:05
Title
01:30
Preparing Microwells to Receive Cells
02:58
Preparing Cells
04:48
Spheroid Formation
08:50
Results: Spheroid Harvest
10:14
Conclusion
Summary
Automatic Translation
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Automatic Translation
我们引进的粒度均匀,并组成控制肿瘤球体的协议大数代(几千到几十万),使用市售微孔板。
Tags
Tumor Spheroids
3D Cell Culture
Microwell Plates
AggreWell
High-throughput
Reproducibility
Cell Aggregation
Tumor Biology
Therapeutic Screening
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